PXD056475-2

PXD056475 is an original dataset announced via ProteomeXchange.

Title	Human CD8 T cell IL2 deprivation time course
Description	IL2 deprivation time course for day 8 human CD8 T cells activated for 3 days with TransAct T-cell activation beads. PBMC (Cambridge Bio) were defrosted and CD8+ T-cells isolated using a human CD8+ T-cell isolation kit. CD8+ T-cells were activated at 1 x 106 cells/mL with 1 in 100 TransAct T-cell activation beads (Miltenyi) and 20 ng/mL IL-2 in complete RMPI (RMPI 1640, 10% FBS, penicillin/streptomycin) for three days. Cells were then washed out of activation and then maintained in complete RMPI with 20 ng/mL IL-2 for five days. To remove IL-2 for IL-2 deprivation, cells were washed twice in pre-warmed media, then replated in complete media without IL-2. Cells were deprived of IL-2 for 1hr, 3hrs, 5hrs, 8hrs, 24hrs or 48hrs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:13.039.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Brenes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-03 05:35:26	ID requested
1	2024-10-11 05:08:10	announced
⏵ 2	2024-10-22 07:01:13	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: DIA, human, CD8,T cells

Doreen A Cantrell
contact affiliation	Cell Signalling & Immunology, University of Dundee
contact email	d.a.cantrell@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliation	Centre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh
contact email	ajbrenesmurillo@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD056475

PRIDE project URI

• PRIDE
  1. PXD056475
     1. Label: PRIDE project
     2. Name: Human CD8 T cell IL2 deprivation time course