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PXD056475-1

PXD056475 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman CD8 T cell IL2 deprivation time course
DescriptionIL2 deprivation time course for day 8 human CD8 T cells activated for 3 days with TransAct T-cell activation beads. PBMC (Cambridge Bio) were defrosted and CD8+ T-cells isolated using a human CD8+ T-cell isolation kit. CD8+ T-cells were activated at 1 x 106 cells/mL with 1 in 100 TransAct T-cell activation beads (Miltenyi) and 20 ng/mL IL-2 in complete RMPI (RMPI 1640, 10% FBS, penicillin/streptomycin) for three days. Cells were then washed out of activation and then maintained in complete RMPI with 20 ng/mL IL-2 for five days. To remove IL-2 for IL-2 deprivation, cells were washed twice in pre-warmed media, then replated in complete media without IL-2. Cells were deprived of IL-2 for 1hr, 3hrs, 5hrs, 8hrs, 24hrs or 48hrs.
HostingRepositoryPRIDE
AnnounceDate2024-10-11
AnnouncementXMLSubmission_2024-10-11_05:08:09.454.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlejandro Brenes
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-03 05:35:26ID requested
12024-10-11 05:08:10announced
22024-10-22 07:01:13announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DIA, human, CD8,T cells
Contact List
Doreen A Cantrell
contact affiliationCell Signalling & Immunology, University of Dundee
contact emaild.a.cantrell@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliationCentre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh
contact emailajbrenesmurillo@dundee.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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