PXD056067-2

PXD056067 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein expression in heated rat lens using shotgun proteomics
Description	Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy-based global proteomic approach was applied to investigate cataract-inducing factors in lens cultured at normal (35.0˚C) and slightly warmer (37.5˚C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0˚C and 37.5˚C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0˚C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5˚C. Protein expression changes in rat lenses cultured at 37.5˚C were examined using a label-free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.
HostingRepository	jPOST
AnnounceDate	2024-09-22
AnnouncementXML	Submission_2024-12-08_18:29:46.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroko Otake
SpeciesList	scientific name: Rattus; NCBI TaxID: 10114;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-21 22:50:29	ID requested
1	2024-09-21 23:32:14	announced
⏵ 2	2024-12-08 18:29:47	announced	2024-12-09: Updated PubMed.

Publication List

Otake H, Masuda S, Yamamoto T, Miyata Y, Nakazawa Y, Yamamoto N, Taga A, Sasaki H, Nagai N, Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics. Mol Med Rep, 31(1):(2025) [pubmed]

Otake H, Masuda S, Yamamoto T, Miyata Y, Nakazawa Y, Yamamoto N, Taga A, Sasaki H, Nagai N, Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics. Mol Med Rep, 31(1):(2025) [pubmed]

Keyword List

submitter keyword: lens, temperature, shotgun proteomics, cataract, protein

submitter keyword: lens, temperature, shotgun proteomics, cataract, protein

Contact List

Tetsushi Yamamoto
lab head
Hiroko Otake
contact affiliation	Kindai University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003374/