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  <ChangeLog>
    <ChangeLogEntry date="2024-12-09">Updated PubMed.</ChangeLogEntry>
  </ChangeLog>
  <DatasetSummary announceDate="2024-09-22" hostingRepository="jPOST" title="Protein expression in heated rat lens using shotgun proteomics">
    <Description>Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy-based global proteomic approach was applied to investigate cataract-inducing factors in lens cultured at normal (35.0&#x2DA;C) and slightly warmer (37.5&#x2DA;C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0&#x2DA;C and 37.5&#x2DA;C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0&#x2DA;C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5&#x2DA;C. Protein expression changes in rat lenses cultured at 37.5&#x2DA;C were examined using a label-free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.</Description>
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      <cvParam cvRef="MS" accession="MS:1002854" name="Peer-reviewed dataset"/>
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      <cvParam accession="MS:1001919" cvRef="MS" name="ProteomeXchange accession number" value="PXD056067"/>
      <cvParam accession="MS:1001921" cvRef="MS" name="ProteomeXchange accession number version number" value="2"/>
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    <DatasetIdentifier>
      <cvParam accession="MS:1002632" cvRef="MS" name="jPOST dataset identifier" value="JPST003374"/>
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    <DatasetOrigin>
      <cvParam cvRef="MS" accession="MS:1002868" name="Original data"/>
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  <SpeciesList>
    <Species>
      <cvParam cvRef="MS" accession="MS:1001469" name="taxonomy: scientific name" value="Rattus"/>
      <cvParam cvRef="MS" accession="MS:1001467" name="taxonomy: NCBI TaxID" value="10114"/>
    </Species>
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    <Instrument id="Instrument_1">
      <cvParam cvRef="MS" accession="MS:1000449" name="LTQ Orbitrap"/>
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    <cvParam cvRef="MOD" accession="MOD:01060" name="S-carboxamidomethyl-L-cysteine"/>
    <cvParam cvRef="MOD" accession="MOD:00719" name="L-methionine sulfoxide"/>
  </ModificationList>
  <ContactList>
    <Contact id="project_submitter">
      <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Hiroko Otake"/>
      <cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="Kindai University"/>
      <cvParam cvRef="MS" accession="MS:1002037" name="dataset submitter"/>
    </Contact>
    <Contact id="project_lab_head">
      <cvParam cvRef="MS" accession="MS:1002332" name="lab head"/>
      <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Tetsushi Yamamoto"/>
    </Contact>
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    <Publication id="PMID39540352">
      <cvParam cvRef="MS" accession="MS:1000879" name="PubMed identifier" value="39540352"/>
      <cvParam cvRef="MS" accession="MS:1002866" name="Reference" value="Otake H, Masuda S, Yamamoto T, Miyata Y, Nakazawa Y, Yamamoto N, Taga A, Sasaki H, Nagai N Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics. Mol Med Rep. 2025 Jan;31(1)"/>
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    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="lens, temperature, shotgun proteomics, cataract, protein"/>
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      <cvParam cvRef="MS" accession="MS:1002852" name="Dataset FTP location" value="ftp://ftp.jpostdb.org/JPST003374/"/>
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