PXD055969-1

PXD055969 is an original dataset announced via ProteomeXchange.

Title	The influence of amniotic membrane proteins delivered directly or through hydrogel platforms on corneal regeneration
Description	Purpose: This study has been aimed to investigate corneal wound healing facilitated by two hydrogels loaded with proteins derived from human amniotic membranes (AMs). Methods: Alkaline burns (8 mm diameter) were induced in the corneas of anesthetized male New Zealand White rabbits (n=44) using circular filter papers soaked in 1 M NaOH for 60 s. The wounds were rinsed immediately with a balanced salt solution, and rabbits received different treatments: 1) no treatment; 2) AM transplantation; 3) a dynamic hyaluronic acid hydrogel based on gold thiolate, and 4) a physically cross-linked ocular insert hydrogel, both loaded with AM protein extracts. The contralateral uninjured eye served as the control. Subsequently, wound area and proportion of healed corneas were assessed using microphotographs. Additionally, corneal histology was evaluated by haematoxylin–eosin and Masson's trichrome staining, examining epithelial and stromal thickness, endothelial layer, and inflammatory infiltration in the early (day 2) and late (day 28) phases of healing. Results: Animals treated with hydrogel (treatments 3 and 4) demonstrated higher corneal wound closure frequencies on day 14 (44.4% and 55.5%, respectively) compared to untreated controls (33.3%). Histologically, abnormal re-epithelialization and alterations in epithelial layer junctions were observed, with no significant differences in epithelial thickness. Endothelial damage correlated with significant thinning (p=0.001), and treatments 2 and 3 showed significant differences in inflammatory infiltrate (p=0.01). Conclusions: Application of new biocompatible hydrogels onto the ocular surface, synthesized to release proteins from AMs, may aid in closing corneal wounds caused by caustic burns. The aggressive nature of burns hinders detection of differences in wound area between treatments. Improving adhesiveness of solid hydrogel could enhance outcomes.
HostingRepository	PRIDE
AnnounceDate	2025-02-11
AnnouncementXML	Submission_2025-02-11_01:57:03.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mikel Azkargorta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-18 02:11:00	ID requested
⏵ 1	2025-02-11 01:57:03	announced

Dataset with its publication pending

submitter keyword: wound healing, amniotic membrane,hydrogel inserts, cornea, rabbit

Felix Elortza
contact affiliation	Proteomics Platform CIC bioGUNE Bizkaia Tech. Par Build. 800 48160 Derio (Spain)
contact email	felortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliation	Proteomics Platform CIC bioGUNE
contact email	mazkargorta@cicbiogune.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055969
     1. Label: PRIDE project
     2. Name: The influence of amniotic membrane proteins delivered directly or through hydrogel platforms on corneal regeneration