Purpose: This study has been aimed to investigate corneal wound healing facilitated by two hydrogels loaded with proteins derived from human amniotic membranes (AMs). Methods: Alkaline burns (8 mm diameter) were induced in the corneas of anesthetized male New Zealand White rabbits (n=44) using circular filter papers soaked in 1 M NaOH for 60 s. The wounds were rinsed immediately with a balanced salt solution, and rabbits received different treatments: 1) no treatment; 2) AM transplantation; 3) a dynamic hyaluronic acid hydrogel based on gold thiolate, and 4) a physically cross-linked ocular insert hydrogel, both loaded with AM protein extracts. The contralateral uninjured eye served as the control. Subsequently, wound area and proportion of healed corneas were assessed using microphotographs. Additionally, corneal histology was evaluated by haematoxylin–eosin and Masson's trichrome staining, examining epithelial and stromal thickness, endothelial layer, and inflammatory infiltration in the early (day 2) and late (day 28) phases of healing. Results: Animals treated with hydrogel (treatments 3 and 4) demonstrated higher corneal wound closure frequencies on day 14 (44.4% and 55.5%, respectively) compared to untreated controls (33.3%). Histologically, abnormal re-epithelialization and alterations in epithelial layer junctions were observed, with no significant differences in epithelial thickness. Endothelial damage correlated with significant thinning (p=0.001), and treatments 2 and 3 showed significant differences in inflammatory infiltrate (p=0.01). Conclusions: Application of new biocompatible hydrogels onto the ocular surface, synthesized to release proteins from AMs, may aid in closing corneal wounds caused by caustic burns. The aggressive nature of burns hinders detection of differences in wound area between treatments. Improving adhesiveness of solid hydrogel could enhance outcomes.