PXD055778-2

PXD055778 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling of trans-cinnamic acid 4-hydroxylase in Petunia inflata petal protoplasts
Description	Proximity-dependent labeling allows untargeted determination of both direct and indirect protein interactions in vivo, and therefore stands as an attractive alternative to targeted binary assays for determining global proteinprotein interaction networks. We used TurboID-based proximity labeling to study protein interaction networks of the core phenylpropanoid and anthocyanin pathways in petunia. To do so, we coupled the endoplasmic reticulum (ER) membrane anchored cytochrome P450 cinnamic acid 4-hydroxylase (C4H, CYP73A412) from Petunia inflata to TurboID and expressed it in protoplasts derived from anthocyanin-rich petunia petals. We identified multiple soluble enzymes from the late anthocyanin pathway among enriched proteins, along with other C4H isoforms, and other ER membrane anchored CYPs. Several of these interactions were subsequently confirmed by bimolecular fluorescence complementation (BiFC).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:58:00.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Silas Mellor
SpeciesList	scientific name: Petunia integrifolia subsp. inflata; NCBI TaxID: 212142;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-11 04:59:28	ID requested
1	2024-09-11 07:06:46	announced
⏵ 2	2024-10-22 06:58:01	announced	2024-10-22: Updated project metadata.

10.3389/FPLS.2024.1295750;

submitter keyword: anthocyanins, proximity labeling, C4H,petunia, plants

Tomas Laursen
contact affiliation	Department of Plant and Environmental Sciences, Section for Plant Biochemistry, Dynamic Metabolons Group
contact email	tola@plen.ku.dk
lab head
Silas Mellor
contact affiliation	University of Copenhagen
contact email	silasmellor@plen.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055778
     1. Label: PRIDE project
     2. Name: Proximity labeling of trans-cinnamic acid 4-hydroxylase in Petunia inflata petal protoplasts