PXD055778 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximity labeling of trans-cinnamic acid 4-hydroxylase in Petunia inflata petal protoplasts |
Description | Proximity-dependent labeling allows untargeted determination of both direct and indirect protein interactions in vivo, and therefore stands as an attractive alternative to targeted binary assays for determining global proteinprotein interaction networks. We used TurboID-based proximity labeling to study protein interaction networks of the core phenylpropanoid and anthocyanin pathways in petunia. To do so, we coupled the endoplasmic reticulum (ER) membrane anchored cytochrome P450 cinnamic acid 4-hydroxylase (C4H, CYP73A412) from Petunia inflata to TurboID and expressed it in protoplasts derived from anthocyanin-rich petunia petals. We identified multiple soluble enzymes from the late anthocyanin pathway among enriched proteins, along with other C4H isoforms, and other ER membrane anchored CYPs. Several of these interactions were subsequently confirmed by bimolecular fluorescence complementation (BiFC). |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-11 |
AnnouncementXML | Submission_2024-09-11_07:06:46.139.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Silas Mellor |
SpeciesList | scientific name: Petunia integrifolia subsp. inflata; NCBI TaxID: 212142; |
ModificationList | iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-11 04:59:28 | ID requested | |
⏵ 1 | 2024-09-11 07:06:46 | announced | |
2 | 2024-10-22 06:58:01 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: anthocyanins, proximity labeling, C4H,petunia, plants |
Contact List
Tomas Laursen |
contact affiliation | Department of Plant and Environmental Sciences, Section for Plant Biochemistry, Dynamic Metabolons Group |
contact email | tola@plen.ku.dk |
lab head | |
Silas Mellor |
contact affiliation | University of Copenhagen |
contact email | silasmellor@plen.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055778
- Label: PRIDE project
- Name: Proximity labeling of trans-cinnamic acid 4-hydroxylase in Petunia inflata petal protoplasts