PXD054637-1

PXD054637 is an original dataset announced via ProteomeXchange.

Title	SILAC proteomics on MG132-induced nuclear aggregates
Description	H1299 cells were labelled either with light (Lys0/Arg0) or heavy (Lys8/Arg10) amino acids and were either unstressed (light), stressed (heavy) with MG132 (15hrs) or stressed with MG132 and allowed to recover for 8hrs (light). Equal number of unstressed and MG132 stressed cells or MG132 stressed cells and cells after 8hrs recovery were mixed before isolation of nuclear inclusions (N-Lauroylsarcosine insoluble inclusions). Isolated inclusions were solubilised in 5% SDS before trypsin digestion (10g of sample) in columns using the universal proteomics sample preparation kit ProtiFi according to manufacturer’s instructions before eluted peptides were analysed by mass-spectrometry. Quantitation of data from 3 independent experiments.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_23:34:19.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuelle Mouton Barbosa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-06 05:33:36	ID requested
⏵ 1	2025-05-06 23:34:20	announced

Brunello L, Polanowska J, Le Tareau L, Maghames C, Georget V, Guette C, Chaoui K, Balor S, O'Donohue MF, Bousquet MP, Gleizes PE, Xirodimas DP, A nuclear protein quality control system for elimination of nucleolus-related inclusions. EMBO J, 44(3):801-823(2025) [pubmed]

10.1038/s44318-024-00333-9;

submitter keyword: nucleolus, nuclear inclusions,SILAC, proteasome, MG132

Odile Schiltz
contact affiliation	Institut de Pharmacologie et de Biologie Structurale, CNRS, Université Paul Sabatier (UPS), Université de Toulouse, Toulouse, 31000 France.
contact email	odile.schiltz@ipbs.fr
lab head
Emmanuelle Mouton Barbosa
contact affiliation	IPBS CNRS
contact email	emmanuelle.mouton@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054637

PRIDE project URI

• PRIDE
  1. PXD054637
     1. Label: PRIDE project
     2. Name: SILAC proteomics on MG132-induced nuclear aggregates