H1299 cells were labelled either with light (Lys0/Arg0) or heavy (Lys8/Arg10) amino acids and were either unstressed (light), stressed (heavy) with MG132 (15hrs) or stressed with MG132 and allowed to recover for 8hrs (light). Equal number of unstressed and MG132 stressed cells or MG132 stressed cells and cells after 8hrs recovery were mixed before isolation of nuclear inclusions (N-Lauroylsarcosine insoluble inclusions). Isolated inclusions were solubilised in 5% SDS before trypsin digestion (10g of sample) in columns using the universal proteomics sample preparation kit ProtiFi according to manufacturer’s instructions before eluted peptides were analysed by mass-spectrometry. Quantitation of data from 3 independent experiments.