PXD054388-2

PXD054388 is an original dataset announced via ProteomeXchange.

Title	Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress
Description	Human development relies on the correct replication, maintenance and segregation of our genetic blueprints. How these processes are monitored across embryonic lineages, and why genomic mosaicism varies during development remain unknown. Using pluripotent stem cells, we identify that several patterning signals –including WNT, BMP and FGF– converge into the modulation of DNA replication stress and damage during S-phase, which in turn controls chromosome segregation fidelity in mitosis. We show that the WNT and BMP signals protect from excessive origin firing, DNA damage and chromosome missegregation derived from stalled forks in pluripotency. Cell signalling control of chromosome segregation declines during lineage specification into the three germ layers, but re-emerges in neural progenitors. In particular, we find that the neurogenic factor FGF2 induces DNA replication stress-mediated chromosome missegregation during the onset of neurogenesis, which could provide a rationale for the elevated chromosomal mosaicism of the developing brain. Our results highlight roles for morphogens and cellular identity in genome maintenance that contribute to somatic mosaicism during mammalian development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:52:01.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anchel de Jaime Soguero
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 06:02:59	ID requested
1	2024-07-31 06:33:13	announced
⏵ 2	2024-10-22 06:52:01	announced	2024-10-22: Updated project metadata.

10.1101/2023.07.18.549463;

submitter keyword: Cellular signaling/ Chromosomal mosaicism/ genome stability/ Embryonic development/ Pluripotent stem cells/ DNA replication stress/ Linage specifications

Prof. Sergio P. Acebron
contact affiliation	Center for Organismal Studies (COS) Heidelberg, University of Heidelberg (Germany)
contact email	sergio.acebron@cos.uni-heidelberg.de
lab head
Anchel de Jaime Soguero
contact affiliation	Center for Organismal Studies (COS) Heidelberg, University of Heidelberg
contact email	anchel.dejaime@cos.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD054388

PRIDE project URI

• PRIDE
  1. PXD054388
     1. Label: PRIDE project
     2. Name: Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress