PXD054388 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress |
Description | Human development relies on the correct replication, maintenance and segregation of our genetic blueprints. How these processes are monitored across embryonic lineages, and why genomic mosaicism varies during development remain unknown. Using pluripotent stem cells, we identify that several patterning signals –including WNT, BMP and FGF– converge into the modulation of DNA replication stress and damage during S-phase, which in turn controls chromosome segregation fidelity in mitosis. We show that the WNT and BMP signals protect from excessive origin firing, DNA damage and chromosome missegregation derived from stalled forks in pluripotency. Cell signalling control of chromosome segregation declines during lineage specification into the three germ layers, but re-emerges in neural progenitors. In particular, we find that the neurogenic factor FGF2 induces DNA replication stress-mediated chromosome missegregation during the onset of neurogenesis, which could provide a rationale for the elevated chromosomal mosaicism of the developing brain. Our results highlight roles for morphogens and cellular identity in genome maintenance that contribute to somatic mosaicism during mammalian development. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-31 |
AnnouncementXML | Submission_2024-07-31_06:33:12.766.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anchel de Jaime Soguero |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-30 06:02:59 | ID requested | |
⏵ 1 | 2024-07-31 06:33:13 | announced | |
2 | 2024-10-22 06:52:01 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Cellular signaling/ Chromosomal mosaicism/ genome stability/ Embryonic development/ Pluripotent stem cells/ DNA replication stress/ Linage specifications |
Contact List
Prof. Sergio P. Acebron |
contact affiliation | Center for Organismal Studies (COS) Heidelberg, University of Heidelberg (Germany) |
contact email | sergio.acebron@cos.uni-heidelberg.de |
lab head | |
Anchel de Jaime Soguero |
contact affiliation | Center for Organismal Studies (COS) Heidelberg, University of Heidelberg |
contact email | anchel.dejaime@cos.uni-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054388
- Label: PRIDE project
- Name: Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress