PXD053980-2

PXD053980 is an original dataset announced via ProteomeXchange.

Title	Huntingtin contains an ubiquitin-binding domain and regulates lysosomal targeting of mitochondrial and RNA-binding proteins
Description	Evaluating the identity of ubiquitinated and ubiquitin-associated proteins tandem co-purifying non-covalently with wild-type (17 glutamine) human Huntingtin fragment, amino acids 1-502 C-terminally tagged with the HA peptide epitope, basally expressed from the CMV promoter within vector pcDNA3.1 transiently transfected into striatal precursor cell line St14A, significantly elevated above cells transfected with empty pcDNA3.1 vector negative control, in cells co-transfected with human FLAG-ubiquitin.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:59.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clinton Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-16 04:35:00	ID requested
1	2024-07-31 07:50:32	announced
⏵ 2	2024-10-22 06:51:59	announced	2024-10-22: Updated project metadata.

10.1073/PNAS.2319091121;

submitter keyword: mitophagy,Huntington’s Disease, selective autophagy, RNA-binding proteins, Huntingtin, Ubiquitin-binding domain

Joan S. Steffan
contact affiliation	Department of Psychiatry and Human Behavior University of California, Irvine Irvine, CA 92697, USA
contact email	jssteffa@uci.edu
lab head
Clinton Yu
contact affiliation	University of California, Irvine
contact email	clinton.yu@uci.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD053980

PRIDE project URI

• PRIDE
  1. PXD053980
     1. Label: PRIDE project
     2. Name: Huntingtin contains an ubiquitin-binding domain and regulates lysosomal targeting of mitochondrial and RNA-binding proteins