PXD053980 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Huntingtin contains an ubiquitin-binding domain and regulates lysosomal targeting of mitochondrial and RNA-binding proteins |
Description | Evaluating the identity of ubiquitinated and ubiquitin-associated proteins tandem co-purifying non-covalently with wild-type (17 glutamine) human Huntingtin fragment, amino acids 1-502 C-terminally tagged with the HA peptide epitope, basally expressed from the CMV promoter within vector pcDNA3.1 transiently transfected into striatal precursor cell line St14A, significantly elevated above cells transfected with empty pcDNA3.1 vector negative control, in cells co-transfected with human FLAG-ubiquitin. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-31 |
AnnouncementXML | Submission_2024-07-31_07:50:31.999.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Clinton Yu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-16 04:35:00 | ID requested | |
⏵ 1 | 2024-07-31 07:50:32 | announced | |
2 | 2024-10-22 06:51:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: mitophagy,Huntington’s Disease, selective autophagy, RNA-binding proteins, Huntingtin, Ubiquitin-binding domain |
Contact List
Joan S. Steffan |
contact affiliation | Department of Psychiatry and Human Behavior University of California, Irvine Irvine, CA 92697, USA |
contact email | jssteffa@uci.edu |
lab head | |
Clinton Yu |
contact affiliation | University of California, Irvine |
contact email | clinton.yu@uci.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD053980 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053980
- Label: PRIDE project
- Name: Huntingtin contains an ubiquitin-binding domain and regulates lysosomal targeting of mitochondrial and RNA-binding proteins