PXD053813-2

PXD053813 is an original dataset announced via ProteomeXchange.

Title	ST8Sia2 polysialyltransferase protects against infection by Trypanosoma cruzi
Description	Glycosylation is one of the most structurally and functionally diverse co- and post-translational modifications in a cell. Addition and removal of glycans, especially to proteins and lipids, characterize this process which have important implications in several biological processes. In mammals, the repeated enzymatic addition of a sialic acid unit to underlying sialic acids (Sia) by polysialyltransferases, including ST8Sia2, leads to the formation of a sugar polymer called polysialic acid (polySia). The functional relevance of polySia has been extensively demonstrated in the nervous system. However, the role of polysialylation in infection is still poorly explored. Previous reports have shown that Trypanosoma cruzi (T. cruzi), a flagellated parasite that causes Chagas disease (CD), changes host sialylation of glycoproteins. To understand the role of host polySia during T. cruzi infection, we used a combination of in silico and experimental tools. We observed that T. cruzi reduces both the expression of the ST8Sia2 and the polysialylation of target substrates. We also found that chemical and genetic inhibition of host ST8Sia2 increased the parasite load in mammalian cells. For the first time, we demonstrated that modulating host polysialylation may induce oxidative stress, creating a microenvironment that favors T. cruzi survival and infection. These findings suggest a novel approach to interfere with parasite infections through modulation of host polysialylation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:39.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janaina Silva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-09 18:08:05	ID requested
1	2024-09-02 11:44:51	announced
⏵ 2	2024-10-22 06:57:39	announced	2024-10-22: Updated project metadata.

10.1371/JOURNAL.PNTD.0012454;

submitter keyword: ST8Sia2, polysialyltransferase, Trypanosoma cruzi

Giuseppe Palmisano
contact affiliation	GlycoProteomics Laboratory, Department of Parasitology, ICB, University of São Paulo, Sao Paulo, Brazil
contact email	palmisano.gp@gmail.com
lab head
Janaina Silva
contact affiliation	Universidade de Sao Paulo
contact email	janainamace@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053813
     1. Label: PRIDE project
     2. Name: ST8Sia2 polysialyltransferase protects against infection by Trypanosoma cruzi