PXD053813 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ST8Sia2 polysialyltransferase protects against infection by Trypanosoma cruzi |
Description | Glycosylation is one of the most structurally and functionally diverse co- and post-translational modifications in a cell. Addition and removal of glycans, especially to proteins and lipids, characterize this process which have important implications in several biological processes. In mammals, the repeated enzymatic addition of a sialic acid unit to underlying sialic acids (Sia) by polysialyltransferases, including ST8Sia2, leads to the formation of a sugar polymer called polysialic acid (polySia). The functional relevance of polySia has been extensively demonstrated in the nervous system. However, the role of polysialylation in infection is still poorly explored. Previous reports have shown that Trypanosoma cruzi (T. cruzi), a flagellated parasite that causes Chagas disease (CD), changes host sialylation of glycoproteins. To understand the role of host polySia during T. cruzi infection, we used a combination of in silico and experimental tools. We observed that T. cruzi reduces both the expression of the ST8Sia2 and the polysialylation of target substrates. We also found that chemical and genetic inhibition of host ST8Sia2 increased the parasite load in mammalian cells. For the first time, we demonstrated that modulating host polysialylation may induce oxidative stress, creating a microenvironment that favors T. cruzi survival and infection. These findings suggest a novel approach to interfere with parasite infections through modulation of host polysialylation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-02 |
AnnouncementXML | Submission_2024-09-02_11:44:51.512.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Janaina Silva |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-09 18:08:05 | ID requested | |
⏵ 1 | 2024-09-02 11:44:51 | announced | |
2 | 2024-10-22 06:57:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: ST8Sia2, polysialyltransferase, Trypanosoma cruzi |
Contact List
Giuseppe Palmisano |
contact affiliation | GlycoProteomics Laboratory, Department of Parasitology, ICB, University of São Paulo, Sao Paulo, Brazil |
contact email | palmisano.gp@gmail.com |
lab head | |
Janaina Silva |
contact affiliation | Universidade de Sao Paulo |
contact email | janainamace@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD053813 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053813
- Label: PRIDE project
- Name: ST8Sia2 polysialyltransferase protects against infection by Trypanosoma cruzi