PXD053811-2

PXD053811 is an original dataset announced via ProteomeXchange.

Title	Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses
Description	Mitosis in early embryos often proceeds at a rapid pace, but how this pace is achieved is not understood. Here we show that cyclin B3 is the dominant driver of rapid embryonic mitoses in the C. elegans embryo. Cyclins B1 and B2 support slow mitosis (NEBD to Anaphase ~600s) but the presence of cyclin B3 dominantly drives the ~3-fold faster mitosis observed in wildtype. Multiple mitotic events are slowed down in Cyclin B1&B2-driven mitosis and cyclin B3-associated Cdk1 H1 kinase activity is ~25-fold more active than cyclin B1-associated Cdk1. Addition of cyclin B1 to fast cyclin B3-only mitosis introduces an ~60s delay between completion of chromosome alignment and anaphase onset; this delay, which is important for segregation fidelity, is dependent on inhibitory phosphorylation of the anaphase activator Cdc20. Thus, cyclin B3 dominance, coupled to a cyclin B1-dependent delay that acts via Cdc20 phosphorylation, sets the rapid pace and ensures mitotic fidelity in the early C. elegans embryo.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:44.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pablo Lara-Gonzalez
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-09 16:33:51	ID requested
1	2024-07-29 00:09:53	announced
⏵ 2	2024-10-22 06:51:44	announced	2024-10-22: Updated project metadata.

10.1083/JCB.202308034;

submitter keyword: Mitosis, Meiosis, Cdk1, cell division, cyclin B1, Cdc20, cyclin B3, APC/C, cyclin B2, embryonic development

Pablo Lara-Gonzalez
contact affiliation	Department of Developmental and Cell Biology Charlie Dunlop School of Biological Sciences University of California Irvine Irvine CA 92697 United States
contact email	plaragon@uci.edu
lab head
Pablo Lara-Gonzalez
contact affiliation	University of California Irvine
contact email	plaragon@uci.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD053811

PRIDE project URI

• PRIDE
  1. PXD053811
     1. Label: PRIDE project
     2. Name: Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses