PXD053811 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses |
Description | Mitosis in early embryos often proceeds at a rapid pace, but how this pace is achieved is not understood. Here we show that cyclin B3 is the dominant driver of rapid embryonic mitoses in the C. elegans embryo. Cyclins B1 and B2 support slow mitosis (NEBD to Anaphase ~600s) but the presence of cyclin B3 dominantly drives the ~3-fold faster mitosis observed in wildtype. Multiple mitotic events are slowed down in Cyclin B1&B2-driven mitosis and cyclin B3-associated Cdk1 H1 kinase activity is ~25-fold more active than cyclin B1-associated Cdk1. Addition of cyclin B1 to fast cyclin B3-only mitosis introduces an ~60s delay between completion of chromosome alignment and anaphase onset; this delay, which is important for segregation fidelity, is dependent on inhibitory phosphorylation of the anaphase activator Cdc20. Thus, cyclin B3 dominance, coupled to a cyclin B1-dependent delay that acts via Cdc20 phosphorylation, sets the rapid pace and ensures mitotic fidelity in the early C. elegans embryo. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-29 |
AnnouncementXML | Submission_2024-07-29_00:09:53.482.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pablo Lara-Gonzalez |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-09 16:33:51 | ID requested | |
⏵ 1 | 2024-07-29 00:09:53 | announced | |
2 | 2024-10-22 06:51:44 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Mitosis, Meiosis, Cdk1, cell division, cyclin B1, Cdc20, cyclin B3, APC/C, cyclin B2, embryonic development |
Contact List
Pablo Lara-Gonzalez |
contact affiliation | Department of Developmental and Cell Biology Charlie Dunlop School of Biological Sciences University of California Irvine Irvine CA 92697 United States |
contact email | plaragon@uci.edu |
lab head | |
Pablo Lara-Gonzalez |
contact affiliation | University of California Irvine |
contact email | plaragon@uci.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053811
- Label: PRIDE project
- Name: Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses