PXD052980-1
PXD052980 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | STEAP3 promotes TNBC growth through the FGFR1-mediated activation of PI3K/AKT signaling |
Description | Breast cancer cells were cultured and harvested for proteomic analysis. Cells were lysed using a buffer containing 8 M urea, and proteins were extracted by sonication. The lysate was centrifuged to remove debris, and the supernatant was collected. Protein concentration was determined using the BCA assay. Samples were then reduced with DTT, alkylated with IAA, and digested overnight with trypsin. Peptides were desalted using C18 columns before mass spectrometry analysis. |
HostingRepository | iProX |
AnnounceDate | 2024-06-11 |
AnnouncementXML | Submission_2024-06-10_19:25:09.380.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lifang Yuan |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-06-10 19:23:55 | ID requested | |
⏵ 1 | 2024-06-10 19:25:09 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: STEAP3, TNBC, PI3K-AKT-mTOR, cell proliferation |
Contact List
Lifang Yuan | |
---|---|
contact affiliation | Yantai Yuhuangding hospital, Shandong University, |
contact email | yuanlf2020@mail.sdu.edu.cn |
lab head | |
Lifang Yuan | |
contact affiliation | Yantai Yuhuangding hospital, Shandong University. |
contact email | yuanlf2020@mail.sdu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |