PXD051860-1

PXD051860 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Changes in Protein Fluxes and Gene Expression in Non-Injured Muscle Tissue Distant from an Acute Myotoxic Injury in Male Mice
Description	The response to acute myotoxic injury requires stimulation of local repair mechanisms in the damaged tissue. However, satellite cells in muscle distant from acute injury have been reported to enter a functional state between quiescence and active proliferation. Here, we asked whether protein flux rates are altered in muscle distant from acute local myotoxic injury and how they compare to changes in gene expression from the same tissue. Broad and significant alterations in protein turnover were observed across the proteome in the limb contralateral to injury during the first 10 days after. Interestingly, mRNA changes had almost no correlation with directly measured protein turnover rates. In summary, we show consistent and striking changes in protein flux rates in muscle tissue contralateral to myotoxic injury, with no correlation between changes in mRNA levels and protein synthesis rates. This work motivates further investigation of the mechanisms, including potential neurologic factors, responsible for this distant effect.
HostingRepository	jPOST
AnnounceDate	2024-04-30
AnnouncementXML	Submission_2024-04-29_14:13:33.763.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alec Bizieff
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	unknown modification; unknown modification
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 14:13:30	ID requested
⏵ 1	2024-04-29 14:13:34	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Muscle Damage/Injury
In-Vivo Regeneration
Satellite Cells
Proteomics
Stable Isotope Labeling
Mass Spectrometry
Protein Turnover

submitter keyword: Muscle Damage/Injury

In-Vivo Regeneration

Satellite Cells

Proteomics

Stable Isotope Labeling

Mass Spectrometry

Protein Turnover

Contact List

Alec Bizieff
lab head
Alec Bizieff
contact affiliation	UC Berkeley
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002987/