PXD051855-1

PXD051855 is an original dataset announced via ProteomeXchange.

Title	Mass-spectrometry analysis of vehicle or AKB-9778 treated FOXF2KO and WT human endothelial cells
Description	To validate our results on Foxf2 related Tie2 signaling and explore their transferability to human cells, we studied human iPSC-derived endothelial cells (iECs). FOXF2KO and WT iECs (González-Gallego et al., in preparation) were treated with AKB-9778 or vehicle and assessed by proteomics. Similar to our results in mouse BECs, proteomic analysis revealed a dysregulation of multiple members of the TIE2 signaling pathway in vehicle treated FOXF2KO compared to WT iECs including TIE2 and NOS3. Moreover, treatment with AKB-9778 restored the levels of multiple TIE2 signaling related proteins. Collectively, these findings demonstrate that AKB-9778 rescues the deficiency of TIE2 signaling in human endothelial cells lacking FOXF2. González-Gallego, J. et al. A fully iPSC-derived 3D model of the human blood-brain-barrier for exploring neurovascular disease mechanisms and therapeutic interventions - in preparation, available from editorial office upon request.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_12:04:23.096.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 10:50:06	ID requested
⏵ 1	2025-09-02 12:04:23	announced

Dataset with its publication pending

submitter keyword: small vessel disease,FOXF2, AKB-9778, TIE2, endothelial cell

Stefan F.
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051855
     1. Label: PRIDE project
     2. Name: Mass-spectrometry analysis of vehicle or AKB-9778 treated FOXF2KO and WT human endothelial cells