To validate our results on Foxf2 related Tie2 signaling and explore their transferability to human cells, we studied human iPSC-derived endothelial cells (iECs). FOXF2KO and WT iECs (González-Gallego et al., in preparation) were treated with AKB-9778 or vehicle and assessed by proteomics. Similar to our results in mouse BECs, proteomic analysis revealed a dysregulation of multiple members of the TIE2 signaling pathway in vehicle treated FOXF2KO compared to WT iECs including TIE2 and NOS3. Moreover, treatment with AKB-9778 restored the levels of multiple TIE2 signaling related proteins. Collectively, these findings demonstrate that AKB-9778 rescues the deficiency of TIE2 signaling in human endothelial cells lacking FOXF2. González-Gallego, J. et al. A fully iPSC-derived 3D model of the human blood-brain-barrier for exploring neurovascular disease mechanisms and therapeutic interventions - in preparation, available from editorial office upon request.