PXD050987-2

PXD050987 is an original dataset announced via ProteomeXchange.

Title	FAS Inhibited Proteomics and Phosphoproteomics Profiling of Colorectal Cancer Spheroids shows Activation of Ferroptotic Death Mechanism
Description	Colorectal cancer (CRC) is projected to become the third most diagnosed and third most fatal cancer in the United States by 2024, with early onset CRC on the rise. Research is constantly underway to discover novel therapeutics for the treatment of various cancers to improve patient outcomes and survival rates. Recently, fatty acid synthase (FAS) has become a druggable target of interest for the treatment of many different cancers. FAS inhibitors have been developed over the years with mixed success. One such inhibitor, TVB-2640, has gained popularity for its high specificity for FAS and has even entered a phase 1 clinical trial for the treatment of solid tumors. However, the distinct molecular differences that occur upon inhibiting FAS have yet to be understood. Here, we conduct time course proteomics and phosphoproteomics on analyses HCT 116 and HT-29 CRC spheroids inhibited with either a generation 1 (cerulenin) or generation 2 (TVB-2640) FAS inhibitor. Proteins involved in lipid metabolism and cellular respiration were altered in abundance. It was also observed that proteins and enzymes involved in ferroptosis—an iron mediated form of cell death—were altered. These results show HT-29 spheroids exposed to cerulenin or TVB-2640 are undergoing a ferroptotic death mechanism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:27.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brian Fries
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-26 08:41:45	ID requested
1	2024-08-10 12:38:21	announced
⏵ 2	2024-10-22 06:54:35	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.4c00252;

submitter keyword: Phosphoproteomics, Mass Spectrometry, Colorectal Cancer, Cancer,Proteomics, Data-Independent Acquisition, Spheroids

Amanda B. Hummon
contact affiliation	Department of Chemistry and Biocheimstry at The Ohio State University; Comprehensive Cancer Center, The Ohio State University
contact email	hummon.1@osu.edu
lab head
Brian Fries
contact affiliation	The Ohio State University
contact email	fries.94@osu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050987
     1. Label: PRIDE project
     2. Name: FAS Inhibited Proteomics and Phosphoproteomics Profiling of Colorectal Cancer Spheroids shows Activation of Ferroptotic Death Mechanism