PXD050987 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FAS Inhibited Proteomics and Phosphoproteomics Profiling of Colorectal Cancer Spheroids shows Activation of Ferroptotic Death Mechanism |
Description | Colorectal cancer (CRC) is projected to become the third most diagnosed and third most fatal cancer in the United States by 2024, with early onset CRC on the rise. Research is constantly underway to discover novel therapeutics for the treatment of various cancers to improve patient outcomes and survival rates. Recently, fatty acid synthase (FAS) has become a druggable target of interest for the treatment of many different cancers. FAS inhibitors have been developed over the years with mixed success. One such inhibitor, TVB-2640, has gained popularity for its high specificity for FAS and has even entered a phase 1 clinical trial for the treatment of solid tumors. However, the distinct molecular differences that occur upon inhibiting FAS have yet to be understood. Here, we conduct time course proteomics and phosphoproteomics on analyses HCT 116 and HT-29 CRC spheroids inhibited with either a generation 1 (cerulenin) or generation 2 (TVB-2640) FAS inhibitor. Proteins involved in lipid metabolism and cellular respiration were altered in abundance. It was also observed that proteins and enzymes involved in ferroptosis—an iron mediated form of cell death—were altered. These results show HT-29 spheroids exposed to cerulenin or TVB-2640 are undergoing a ferroptotic death mechanism. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-10 |
AnnouncementXML | Submission_2024-08-10_12:38:20.256.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Brian Fries |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-26 08:41:45 | ID requested | |
⏵ 1 | 2024-08-10 12:38:21 | announced | |
2 | 2024-10-22 06:54:35 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.jproteome.4c00252; |
Fries BD, Hummon AB, FAS Inhibited Proteomics and Phosphoproteomics Profiling of Colorectal Cancer Spheroids Shows Activation of Ferroptotic Death Mechanism. J Proteome Res, 23(9):3904-3916(2024) [pubmed] |
Keyword List
submitter keyword: Phosphoproteomics, Mass Spectrometry, Colorectal Cancer, Cancer,Proteomics, Data-Independent Acquisition, Spheroids |
Contact List
Amanda B. Hummon |
contact affiliation | Department of Chemistry and Biocheimstry at The Ohio State University; Comprehensive Cancer Center, The Ohio State University |
contact email | hummon.1@osu.edu |
lab head | |
Brian Fries |
contact affiliation | The Ohio State University |
contact email | fries.94@osu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050987
- Label: PRIDE project
- Name: FAS Inhibited Proteomics and Phosphoproteomics Profiling of Colorectal Cancer Spheroids shows Activation of Ferroptotic Death Mechanism