PXD050816-2

PXD050816 is an original dataset announced via ProteomeXchange.

Title	Modular DNA origami compartments for the engineering of a protein unfolding and degradation pathway
Description	Within the cell, chemical reactions are often confined and organized through a modular architecture. In this project, a cell-free nanoscale model of the proteasome was analyzed that exploits this compartmentalization principle to perform regulated protein unfolding and degradation. To this end, the protein unfolding machine p97 and the protease chymotrypsin were encapsulated in different DNA nanocompartments, and the sequential unfolding and proteolytic degradation of a model substrate, I3mEos were monitored at different time points via LC-MS/MS-based proteomics using LFQ quantification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:15.477.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 08:01:01	ID requested
1	2024-07-25 09:39:58	announced
⏵ 2	2024-10-22 06:51:15	announced	2024-10-22: Updated project metadata.

10.1038/S41565-024-01738-7;

submitter keyword: directional degradation, DNA origami compartments,Nanofactories

Farnusch Kaschani
contact affiliation	Farnusch Kaschani, Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050816

PRIDE project URI

• PRIDE
  1. PXD050816
     1. Label: PRIDE project
     2. Name: Modular DNA origami compartments for the engineering of a protein unfolding and degradation pathway