PXD050816 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Modular DNA origami compartments for the engineering of a protein unfolding and degradation pathway |
Description | Within the cell, chemical reactions are often confined and organized through a modular architecture. In this project, a cell-free nanoscale model of the proteasome was analyzed that exploits this compartmentalization principle to perform regulated protein unfolding and degradation. To this end, the protein unfolding machine p97 and the protease chymotrypsin were encapsulated in different DNA nanocompartments, and the sequential unfolding and proteolytic degradation of a model substrate, I3mEos were monitored at different time points via LC-MS/MS-based proteomics using LFQ quantification. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-25 |
AnnouncementXML | Submission_2024-07-25_09:39:58.028.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Farnusch Kaschani |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-20 08:01:01 | ID requested | |
⏵ 1 | 2024-07-25 09:39:58 | announced | |
Publication List
Keyword List
submitter keyword: directional degradation, DNA origami compartments,Nanofactories |
Contact List
Farnusch Kaschani |
contact affiliation | Farnusch Kaschani, Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany |
contact email | farnusch.kaschani@uni-due.de |
lab head | |
Farnusch Kaschani |
contact affiliation | University Duisburg-Essen |
contact email | farnusch.kaschani@uni-due.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050816
- Label: PRIDE project
- Name: Modular DNA origami compartments for the engineering of a protein unfolding and degradation pathway