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PXD050816-1

PXD050816 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleModular DNA origami compartments for the engineering of a protein unfolding and degradation pathway
DescriptionWithin the cell, chemical reactions are often confined and organized through a modular architecture. In this project, a cell-free nanoscale model of the proteasome was analyzed that exploits this compartmentalization principle to perform regulated protein unfolding and degradation. To this end, the protein unfolding machine p97 and the protease chymotrypsin were encapsulated in different DNA nanocompartments, and the sequential unfolding and proteolytic degradation of a model substrate, I3mEos were monitored at different time points via LC-MS/MS-based proteomics using LFQ quantification.
HostingRepositoryPRIDE
AnnounceDate2024-07-25
AnnouncementXMLSubmission_2024-07-25_09:39:58.028.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFarnusch Kaschani
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-20 08:01:01ID requested
12024-07-25 09:39:58announced
Publication List
10.1038/S41565-024-01738-7;
Keyword List
submitter keyword: directional degradation, DNA origami compartments,Nanofactories
Contact List
Farnusch Kaschani
contact affiliationFarnusch Kaschani, Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact emailfarnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliationUniversity Duisburg-Essen
contact emailfarnusch.kaschani@uni-due.de
dataset submitter
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Dataset FTP location
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