PXD050763-1

PXD050763 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of the effect of Callyaerin B derivativs on M. tuberculosis H37Rv
Description	The natural cyclopeptide Callyaerin B (CalB) exhibits highly specific antitubercular activity. The aim of this project is to study the proteome dysregulation induced by CalB treatment of M. tuberculosis H37Rv wild type cells using a whole proteome comparison approach. In addition, it has been shown, that the susceptibility of M. tuberculosis H37Rv to CalB is highly dependent on the expression of the putative membrane protein Rv2113. A gene deletion mutation of this protein (Δrv2113) was used to identify unspecific changes in the proteome upon CalB treatment. For this purpose, M. tuberculosis H37Rv wild type or Δrv2113 cells were treated with 31.3 µM CalB (equivalent to a 10-fold MIC90 concentration) at 37 °C for 48 hours and the proteome was compared to a corresponding solvent control (0.31% DMSO)(ACE_0685). As an additional control, the proteosomal changes after treatment of M. tuberculosis H37Rv wild type cells with a modified CalB-derivative CalB_R3β-hIle were studied in a similar setup (ACE_0641).
HostingRepository	PRIDE
AnnounceDate	2024-07-25
AnnouncementXML	Submission_2024-07-25_10:02:39.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-19 05:22:16	ID requested
⏵ 1	2024-07-25 10:02:40	announced

10.1016/j.chembiol.2024.06.002;

Podlesainski D, Adeniyi ET, Gr, ö, ner Y, Schulz F, Krisilia V, Rehberg N, Richter T, Sehr D, Xie H, Simons VE, Kiffe-Delf AL, Kaschani F, Ioerger TR, Kaiser M, Kalscheuer R, The anti-tubercular callyaerins target the Mycobacterium tuberculosis-specific non-essential membrane protein Rv2113. Cell Chem Biol, ():(2024) [pubmed]

submitter keyword: M. tuberculosis H37Rv

Callyaerin B

Cal B

Farnusch Kaschani
contact affiliation	Farnusch Kaschani, Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050763

PRIDE project URI

• PRIDE
  1. PXD050763
     1. Label: PRIDE project
     2. Name: Proteomic study of the effect of Callyaerin B derivativs on M. tuberculosis H37Rv