The natural cyclopeptide Callyaerin B (CalB) exhibits highly specific antitubercular activity. The aim of this project is to study the proteome dysregulation induced by CalB treatment of M. tuberculosis H37Rv wild type cells using a whole proteome comparison approach. In addition, it has been shown, that the susceptibility of M. tuberculosis H37Rv to CalB is highly dependent on the expression of the putative membrane protein Rv2113. A gene deletion mutation of this protein (Δrv2113) was used to identify unspecific changes in the proteome upon CalB treatment. For this purpose, M. tuberculosis H37Rv wild type or Δrv2113 cells were treated with 31.3 µM CalB (equivalent to a 10-fold MIC90 concentration) at 37 °C for 48 hours and the proteome was compared to a corresponding solvent control (0.31% DMSO)(ACE_0685). As an additional control, the proteosomal changes after treatment of M. tuberculosis H37Rv wild type cells with a modified CalB-derivative CalB_R3β-hIle were studied in a similar setup (ACE_0641).