PXD050590-1
PXD050590 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Target deconvolution of SW016789 in human and mouse Beta cells |
| Description | The target of SW016789 is unknown (PMCID: PMC9225822). This compound was identified in a high-throughput screen for insulin secretion modulators and was subsequently discovered to cause hypersecretory stress in beta cells. A photoaffinity probe was designed, Z629, based upon the structure of SW016789. Photo-crosslinking, click chemistry to biotin, and affinity purification with streptavidin-agarose was used to enrich for potential binding partners. Experiments were performed in both mouse MIN6 and human EndoC-BetaH1 beta cells. Enriched proteins were identified based on the ratio of PSMs detected in Z629 vs DMSO treatments. Candidate proteins (>2-fold) were filtered based on a >2-fold enrichment in Z629/DMSO and >1 PSM for Z629 in both MIN6 and EndoC-BetaH1. Pathway analysis indicated a role for the Vdac family of proteins which were previously identified to have an important role in Beta-cells during type 2 diabetes (PMCID: PMC6331340). Vdac1 was therefore chosen for validation studies. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-12-26 |
| AnnouncementXML | Submission_2024-12-26_17:56:46.129.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Emma Doud |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation; Phospho |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-03-13 08:39:28 | ID requested | |
| ⏵ 1 | 2024-12-26 17:56:46 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: pancreatic Beta cells, AP-MS/MS, Vdac |
Contact List
| Michael Kalwat | |
|---|---|
| contact affiliation | Indiana Biosciences Research Institute |
| contact email | mkalwat@indianabiosciences.org |
| lab head | |
| Amber L. Mosley | |
| contact affiliation | Indiana University School of Medicine |
| contact email | almosley@iu.edu |
| lab head | |
| Emma Doud | |
| contact affiliation | IUSM |
| contact email | edoud@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094310/ |
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