PXD050065-2

PXD050065 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cold Atmospheric Plasma Jet Activated Medium Promotes Wound Healing through CK2-mediated ERK and AKT signaling pathways
Description	In this study, we aimed to explore the molecular mechanisms of wound healing affected by CAPJ using the parallel MS-based proteome and phosphoproteome. The keratinocyte, HaCaT, was used as cell mode to treat CAPJ indirectly by employing plasma activated medium (PAM) which was culture medium explored to He/Ar-generated CAPJ following previous study’s procedures. The datasets were integrated and systematically analyzed to dissect molecular and signaling pathway alternation in keratinocytes during PAM intervention. The results showed that PAM treatment significantly activated ERK, CK2 and AKT kinases and the phosphorylation-dependent signaling pathways of PI3K/AKT/mTOR and MEK/ERK were multiaxially disturbed, in which CK2 was suggested to play a coordination role between these two pathways. Collectively, this knowledge allows for a better understanding of how CAPJ regulates keratinocyte signaling during wound closure, and provides new insights into the development of clinical skin wound treatments.
HostingRepository	jPOST
AnnounceDate	2025-02-22
AnnouncementXML	Submission_2025-04-09_05:46:00.648.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miao-Hsia Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-22 23:19:44	ID requested
1	2025-02-21 07:00:32	announced
⏵ 2	2025-04-09 05:46:01	announced	2025-04-09: Updated PubMed.

Publication List

Wu PS, Wong TH, Hou CW, Chu TP, Lee JW, Lou BS, Lin MH, Cold Atmospheric Plasma Jet Promotes Wound Healing Through CK2-Coordinated PI3K/AKT and MAPK Signaling Pathways. Mol Cell Proteomics, 24(5):100962(2025) [pubmed]

Wu PS, Wong TH, Hou CW, Chu TP, Lee JW, Lou BS, Lin MH, Cold Atmospheric Plasma Jet Promotes Wound Healing Through CK2-Coordinated PI3K/AKT and MAPK Signaling Pathways. Mol Cell Proteomics, 24(5):100962(2025) [pubmed]

Keyword List

submitter keyword: HaCaT, Cold Atmospheric Plasma Jet, wound healing, CK2, ERK, phosphorylation

submitter keyword: HaCaT, Cold Atmospheric Plasma Jet, wound healing, CK2, ERK, phosphorylation

Contact List

Miao-Hsia Lin
lab head
Miao-Hsia Lin
contact affiliation	Department of Microbiology, College of Medicine, National Taiwan University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002954/