Updated PubMed. In this study, we aimed to explore the molecular mechanisms of wound healing affected by CAPJ using the parallel MS-based proteome and phosphoproteome. The keratinocyte, HaCaT, was used as cell mode to treat CAPJ indirectly by employing plasma activated medium (PAM) which was culture medium explored to He/Ar-generated CAPJ following previous study’s procedures. The datasets were integrated and systematically analyzed to dissect molecular and signaling pathway alternation in keratinocytes during PAM intervention. The results showed that PAM treatment significantly activated ERK, CK2 and AKT kinases and the phosphorylation-dependent signaling pathways of PI3K/AKT/mTOR and MEK/ERK were multiaxially disturbed, in which CK2 was suggested to play a coordination role between these two pathways. Collectively, this knowledge allows for a better understanding of how CAPJ regulates keratinocyte signaling during wound closure, and provides new insights into the development of clinical skin wound treatments.