PXD049434-2

PXD049434 is an original dataset announced via ProteomeXchange.

Title	2 integrins impose a mechanical checkpoint on macrophage phagocytosis
Description	Phagocytosis is an intensely physical process that depends on the mechanical properties of both the phagocytic cell and its chosen target. Here, we employed differentially deformable hydrogel microparticles to examine the role of cargo rigidity in the regulation of phagocytosis by macrophages. Whereas stiff cargos elicited canonical phagocytic cup formation and rapid engulfment, soft cargos induced an architecturally distinct response, characterized by filamentous actin protrusions at the center of the contact site, slower cup advancement, and frequent phagocytic stalling. Using phosphoproteomics, we identified 2 integrins and their downstream effectors as critical mediators of this mechanically regulated phagocytic switch. Indeed, comparison of wild type and 2 integrin deficient macrophages indicated that integrin signaling acts as a mechanical checkpoint by shaping filamentous actin to enable distinct phagocytic engulfment strategies. Collectively, these results illuminate the molecular logic of leukocyte mechanosensing and reveal potential avenues for modulating phagocyte function in immunotherapeutic contexts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:55:52.273.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuoning Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-16 14:37:18	ID requested
1	2024-09-13 09:44:55	announced
⏵ 2	2024-10-22 06:55:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mouse, LC-MS

Morgan Huse
contact affiliation	Immunology Program, Sloan Kettering Institute, New York, NY, USA.
contact email	husem@mskcc.org
lab head
Zhuoning Li
contact affiliation	MSKCC
contact email	liz2@mskcc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD049434

PRIDE project URI

• PRIDE
  1. PXD049434
     1. Label: PRIDE project
     2. Name: 2 integrins impose a mechanical checkpoint on macrophage phagocytosis