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PXD049434-1

PXD049434 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title2 integrins impose a mechanical checkpoint on macrophage phagocytosis
DescriptionPhagocytosis is an intensely physical process that depends on the mechanical properties of both the phagocytic cell and its chosen target. Here, we employed differentially deformable hydrogel microparticles to examine the role of cargo rigidity in the regulation of phagocytosis by macrophages. Whereas stiff cargos elicited canonical phagocytic cup formation and rapid engulfment, soft cargos induced an architecturally distinct response, characterized by filamentous actin protrusions at the center of the contact site, slower cup advancement, and frequent phagocytic stalling. Using phosphoproteomics, we identified 2 integrins and their downstream effectors as critical mediators of this mechanically regulated phagocytic switch. Indeed, comparison of wild type and 2 integrin deficient macrophages indicated that integrin signaling acts as a mechanical checkpoint by shaping filamentous actin to enable distinct phagocytic engulfment strategies. Collectively, these results illuminate the molecular logic of leukocyte mechanosensing and reveal potential avenues for modulating phagocyte function in immunotherapeutic contexts.
HostingRepositoryPRIDE
AnnounceDate2024-09-13
AnnouncementXMLSubmission_2024-09-13_09:44:55.458.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhuoning Li
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-16 14:37:18ID requested
12024-09-13 09:44:55announced
22024-10-22 06:55:52announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mouse, LC-MS
Contact List
Morgan Huse
contact affiliationImmunology Program, Sloan Kettering Institute, New York, NY, USA.
contact emailhusem@mskcc.org
lab head
Zhuoning Li
contact affiliationMSKCC
contact emailliz2@mskcc.org
dataset submitter
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Dataset FTP location
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