PXD049184-2

PXD049184 is an original dataset announced via ProteomeXchange.

Title	Identification of phosphorylation sites on NAP1/AZI2
Description	We observed smearing for the TBK1-adaptor protein NAP1/AZI2 during mitophagy. To verify whether this smearing was due to phosphorylation by TBK1, we performed an in vitro kinase assay and complemented this with the immunoprecipitation of NAP1 from HAP1, whose genetic background (FIP200 knockout) results in hyperactivation of TBK1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:57.815.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-05 07:56:25	ID requested
1	2024-04-12 04:30:13	announced
⏵ 2	2024-10-22 06:35:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: AZI2, NAP1,Mitophagy, TBK1

Sascha Martens
contact affiliation	University of Vienna, Max Perutz Labs, Department of Biochemistry and Cell Biology, Dr.-Bohr-Gasse 9 / Vienna Biocenter 5, 1030, Vienna, Austria
contact email	sascha.martens@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD049184

PRIDE project URI

• PRIDE
  1. PXD049184
     1. Label: PRIDE project
     2. Name: Identification of phosphorylation sites on NAP1/AZI2