PXD049013-2

PXD049013 is an original dataset announced via ProteomeXchange.

Title	Target identification of usnic acid in bacterial and human cells
Description	Usnic acid is a natural product with versatile biological activities against various organisms. We here utilize a chemical proteomic strategy to gain insights into its target scope in bacterial and human cells. First, we excluded DNA binding as a major reason for its antibacterial activity and second commenced with target profiling which unravelled several metal cofactor-dependent enzymes in both species. This is in line with the metal complexation activities of usnic acid and suggests a polypharmacological mode of action. Interestingly, our synthetic studies revealed a selectivity switch at usnic acid which maintains antibacterial activity but lacks strong cytotoxic effects.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:24.621.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dietrich Mostert
SpeciesList	scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-30 08:21:11	ID requested
1	2024-08-10 12:29:42	announced
⏵ 2	2024-10-22 06:54:25	announced	2024-10-22: Updated project metadata.

10.1039/d4cb00040d;

Ruddell SA, Mostert D, Sieber SA, Target identification of usnic acid in bacterial and human cells. RSC Chem Biol, 5(7):617-621(2024) [pubmed]

submitter keyword: ABPP,Usnic acid

Stephan A. Sieber
contact affiliation	TUM Center for Functional Protein Assemblies (CPA), Department of Chemistry and Chair of Organic Chemistry II, Technical University of Munich, Ernst-Otto-Fischer Strasse 8, 85748 Garching, Germany.
contact email	stephan.sieber@tum.de
lab head
Dietrich Mostert
contact affiliation	Center for Functional Protein Assemblies (CPA), Department of Chemistry, Chair of Organic Chemistry II, Technical University of Munich, 85748 Garching, Germany.
contact email	dietrich.mostert@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD049013

PRIDE project URI

• PRIDE
  1. PXD049013
     1. Label: PRIDE project
     2. Name: Target identification of usnic acid in bacterial and human cells