PXD049013 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Target identification of usnic acid in bacterial and human cells |
Description | Usnic acid is a natural product with versatile biological activities against various organisms. We here utilize a chemical proteomic strategy to gain insights into its target scope in bacterial and human cells. First, we excluded DNA binding as a major reason for its antibacterial activity and second commenced with target profiling which unravelled several metal cofactor-dependent enzymes in both species. This is in line with the metal complexation activities of usnic acid and suggests a polypharmacological mode of action. Interestingly, our synthetic studies revealed a selectivity switch at usnic acid which maintains antibacterial activity but lacks strong cytotoxic effects. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-10 |
AnnouncementXML | Submission_2024-08-10_12:29:41.759.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dietrich Mostert |
SpeciesList | scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-30 08:21:11 | ID requested | |
⏵ 1 | 2024-08-10 12:29:42 | announced | |
2 | 2024-10-22 06:54:25 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1039/d4cb00040d; |
Ruddell SA, Mostert D, Sieber SA, Target identification of usnic acid in bacterial and human cells. RSC Chem Biol, 5(7):617-621(2024) [pubmed] |
Keyword List
submitter keyword: ABPP,Usnic acid |
Contact List
Stephan A. Sieber |
contact affiliation | TUM Center for Functional Protein Assemblies (CPA), Department of Chemistry and Chair of Organic Chemistry II, Technical University of Munich, Ernst-Otto-Fischer Strasse 8, 85748 Garching, Germany. |
contact email | stephan.sieber@tum.de |
lab head | |
Dietrich Mostert |
contact affiliation | Center for Functional Protein Assemblies (CPA), Department of Chemistry, Chair of Organic Chemistry II, Technical University of Munich, 85748 Garching, Germany. |
contact email | dietrich.mostert@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD049013 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049013
- Label: PRIDE project
- Name: Target identification of usnic acid in bacterial and human cells