PXD048658-2

PXD048658 is an original dataset announced via ProteomeXchange.

Title	Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis
Description	The mechanism by which aging induces aortic aneurysm and dissection (AAD) remains unclear. A total of 430 subjects were recruited for screening of differentially expressed plasma microRNAs. We found that miR-1204 was significantly increased in both plasma and aorta of elder patients with AAD, and was positively correlated with age. Cell senescence induced the expression of miR-1204 through p53 interaction with plasmacytoma variant translocation 1, and miR-1204 induced vascular smooth muscle cell (VSMC) senescence to form a positive feedback loop. miR-1204 aggravated angiotensin II-induced AAD formation, and inhibition of miR-1204 attenuated β-aminopropionitrile monofumarate-induced AAD formation. Mechanistically, miR-1204 directly targeted myosin light chain kinase (MYLK) to promote VSMCs to acquire senescence-associated secretory phenotype (SASP) and lose their contractile phenotype. Overexpression of MYLK reversed miR-1204-induced VSMC senescence, SASP and contractile phenotype changes, and the decrease of transforming growth factor-β signaling pathway. Our findings suggest aging aggravates AAD via miR-1204-MYLK signaling axis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:34:56.060.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingsong Ou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-17 17:43:14	ID requested
1	2024-04-01 03:33:11	announced
⏵ 2	2024-10-22 06:34:56	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: aortic aneurysm and dissection

aging

senescence-associated secretory phenotype

microRNA

myosin light chain kinase

Jingsong Ou
contact affiliation	The First Affiliated Hospital, Sun Yat-sen University
contact email	oujs@mail.sysu.edu.cn
lab head
Jingsong Ou
contact affiliation	The First Affiliated Hospital, Sun Yat-sen University
contact email	oujs2000@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048658
     1. Label: PRIDE project
     2. Name: Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis