PXD048658-1

PXD048658 is an original dataset announced via ProteomeXchange.

Title	Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis
Description	The mechanism by which aging induces aortic aneurysm and dissection (AAD) remains unclear. A total of 430 subjects were recruited for screening of differentially expressed plasma microRNAs. We found that miR-1204 was significantly increased in both plasma and aorta of elder patients with AAD, and was positively correlated with age. Cell senescence induced the expression of miR-1204 through p53 interaction with plasmacytoma variant translocation 1, and miR-1204 induced vascular smooth muscle cell (VSMC) senescence to form a positive feedback loop. miR-1204 aggravated angiotensin II-induced AAD formation, and inhibition of miR-1204 attenuated β-aminopropionitrile monofumarate-induced AAD formation. Mechanistically, miR-1204 directly targeted myosin light chain kinase (MYLK) to promote VSMCs to acquire senescence-associated secretory phenotype (SASP) and lose their contractile phenotype. Overexpression of MYLK reversed miR-1204-induced VSMC senescence, SASP and contractile phenotype changes, and the decrease of transforming growth factor-β signaling pathway. Our findings suggest aging aggravates AAD via miR-1204-MYLK signaling axis.
HostingRepository	PRIDE
AnnounceDate	2024-04-01
AnnouncementXML	Submission_2024-04-01_03:33:10.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingsong Ou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-17 17:43:14	ID requested
⏵ 1	2024-04-01 03:33:11	announced
2	2024-10-22 06:34:56	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: aortic aneurysm and dissection

aging

senescence-associated secretory phenotype

microRNA

myosin light chain kinase

Jingsong Ou
contact affiliation	The First Affiliated Hospital, Sun Yat-sen University
contact email	oujs@mail.sysu.edu.cn
lab head
Jingsong Ou
contact affiliation	The First Affiliated Hospital, Sun Yat-sen University
contact email	oujs2000@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD048658

PRIDE project URI

• PRIDE
  1. PXD048658
     1. Label: PRIDE project
     2. Name: Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis