PXD048056-2

PXD048056 is an original dataset announced via ProteomeXchange.

Title	Monocarboxylate transporters facilitate succinate uptake into brown adipocytes
Description	Uptake of circulating succinate by brown adipose tissue (BAT) and beige fat elevates whole-body energy expenditure, counteracts obesity, and antagonizes systemic tissue inflammation in mice. The plasma membrane transporters that facilitate succinate uptake in these adipocytes remain undefined. Here we elucidate a mechanism underlying succinate import into BAT via monocarboxylate transporters (MCTs). We show that succinate transport is strongly dependent on the proportion of it present in the monocarboxylate form. MCTs facilitate monocarboxylate succinate uptake, which is promoted by alkalinization of the cytosol driven by adrenoreceptor stimulation. In brown adipocytes, we show that MCT1 primarily facilitates succinate import. In mice, we show that both acute pharmacological inhibition of MCT1 and congenital depletion of MCT1, decrease succinate uptake into BAT and consequent catabolism. In sum, we define a mechanism of succinate uptake in BAT that underlies its protective activity in mouse models of metabolic disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:20:05.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haopeng Xiao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-21 13:19:22	ID requested
1	2024-01-10 10:54:33	announced
⏵ 2	2024-10-22 06:20:05	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: succinate, brown adipose tissue, thermogenesis, MCT1, BAT

Edward Chouchani
contact affiliation	1 Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA 2 Department of Cell Biology, Harvard Medical School, Boston, MA, USA
contact email	edwardt_chouchani@dfci.harvard.edu
lab head
Haopeng Xiao
contact affiliation	Dana-Farber Cancer Institute/Harvard Medical School
contact email	haopeng_xiao@dfci.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048056
     1. Label: PRIDE project
     2. Name: Monocarboxylate transporters facilitate succinate uptake into brown adipocytes