PXD048056 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Monocarboxylate transporters facilitate succinate uptake into brown adipocytes |
Description | Uptake of circulating succinate by brown adipose tissue (BAT) and beige fat elevates whole-body energy expenditure, counteracts obesity, and antagonizes systemic tissue inflammation in mice. The plasma membrane transporters that facilitate succinate uptake in these adipocytes remain undefined. Here we elucidate a mechanism underlying succinate import into BAT via monocarboxylate transporters (MCTs). We show that succinate transport is strongly dependent on the proportion of it present in the monocarboxylate form. MCTs facilitate monocarboxylate succinate uptake, which is promoted by alkalinization of the cytosol driven by adrenoreceptor stimulation. In brown adipocytes, we show that MCT1 primarily facilitates succinate import. In mice, we show that both acute pharmacological inhibition of MCT1 and congenital depletion of MCT1, decrease succinate uptake into BAT and consequent catabolism. In sum, we define a mechanism of succinate uptake in BAT that underlies its protective activity in mouse models of metabolic disease. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-10 |
AnnouncementXML | Submission_2024-01-10_10:54:32.616.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Haopeng Xiao |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-21 13:19:22 | ID requested | |
⏵ 1 | 2024-01-10 10:54:33 | announced | |
2 | 2024-10-22 06:20:05 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: succinate, brown adipose tissue, thermogenesis, MCT1, BAT |
Contact List
Edward Chouchani |
contact affiliation | 1 Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA 2 Department of Cell Biology, Harvard Medical School, Boston, MA, USA |
contact email | edwardt_chouchani@dfci.harvard.edu |
lab head | |
Haopeng Xiao |
contact affiliation | Dana-Farber Cancer Institute/Harvard Medical School |
contact email | haopeng_xiao@dfci.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048056
- Label: PRIDE project
- Name: Monocarboxylate transporters facilitate succinate uptake into brown adipocytes