PXD047266-1

PXD047266 is an original dataset announced via ProteomeXchange.

Title	High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells
Description	The cell cycle directs a specific sequence of events that leads to the production of two daughter cells. The temporal ordering of the cell cycle is tightly regulated by changes in protein and phosphorylation abundance. Therefore, high-quality data on cell cycle phase-dependent protein and post-translational modification abundance can provide key insights into fundamental aspects of cell physiology. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in non-transformed hTERT-RPE-1 cells using TMT-labelling quantitative mass spectrometry. Compared with previously published datasets, we provide improved temporal resolution and coverage of key cell cycle regulators identifying 401 proteins and 6,528 phosphorylation events exhibiting cell cycle-dependent oscillation. These data are enriched with complementary information to link cycle-dependent abundance dynamics to functional changes. The results of this analysis are available in the Cell Cycle database (CCdb), an in-depth web-based resource for the cell cycle community.
HostingRepository	PRIDE
AnnounceDate	2025-02-03
AnnouncementXML	Submission_2025-02-03_08:31:00.481.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Graeme Benstead-Hume
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 03:54:43	ID requested
⏵ 1	2025-02-03 08:31:00	announced

Dataset with its publication pending

submitter keyword: Phosphoproteomics, Quantitative Mass Spectrometry,Cell Cycle, Databases., Cell Cycle-dependent Oscillation, Proteomics

Jyoti Choudhary
contact affiliation	Functional Proteomics, ICR
contact email	jyoti.choudhary@icr.ac.uk
lab head
Graeme Benstead-Hume
contact affiliation	ICR
contact email	ghume@icr.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD047266

PRIDE project URI

• PRIDE
  1. PXD047266
     1. Label: PRIDE project
     2. Name: High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells