PXD047226-2

PXD047226 is an original dataset announced via ProteomeXchange.

Title	VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity
Description	Synapses are pivotal sites of plasticity and memory formation. Consequently, synapses are energy consumption hotspots susceptible to dysfunction when their energy supplies are perturbed. Mitochondria are stabilized near synapses via the cytoskeleton and provide the local energy required for synaptic plasticity. However, the mechanisms that tether and stabilize mitochondria to support synaptic plasticity are unknown. We identified proteins exclusively tethering mitochondria to actin near postsynaptic spines. We find that VAP, the vesicle-associated membrane protein-associated protein implicated in amyotrophic lateral sclerosis, stabilizes mitochondria via actin near the spines. To test if the VAP-dependent stable mitochondrial compartments can locally support synaptic plasticity, we used two-photon glutamate uncaging for spine plasticity induction and investigated the induced and adjacent uninduced spines. We find VAP functions as a spatial stabilizer of mitochondrial compartments for up to ~60 min and as a spatial ruler determining the ~30 m dendritic segment supported during synaptic plasticity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:15:59.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive; maXis; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-24 09:26:08	ID requested
1	2023-11-27 09:58:06	announced
⏵ 2	2024-10-22 06:15:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: proximity labeling, mitochondria,synaptic plasticity

Julian Langer
contact affiliation	Max Planck Institute of Brain Research
contact email	julian.langer@brain.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047226
     1. Label: PRIDE project
     2. Name: VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity