PXD047226 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity |
Description | Synapses are pivotal sites of plasticity and memory formation. Consequently, synapses are energy consumption hotspots susceptible to dysfunction when their energy supplies are perturbed. Mitochondria are stabilized near synapses via the cytoskeleton and provide the local energy required for synaptic plasticity. However, the mechanisms that tether and stabilize mitochondria to support synaptic plasticity are unknown. We identified proteins exclusively tethering mitochondria to actin near postsynaptic spines. We find that VAP, the vesicle-associated membrane protein-associated protein implicated in amyotrophic lateral sclerosis, stabilizes mitochondria via actin near the spines. To test if the VAP-dependent stable mitochondrial compartments can locally support synaptic plasticity, we used two-photon glutamate uncaging for spine plasticity induction and investigated the induced and adjacent uninduced spines. We find VAP functions as a spatial stabilizer of mitochondrial compartments for up to ~60 min and as a spatial ruler determining the ~30 m dendritic segment supported during synaptic plasticity. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-27 |
AnnouncementXML | Submission_2023-11-27_09:58:06.442.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julian Langer |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive; maXis; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-24 09:26:08 | ID requested | |
⏵ 1 | 2023-11-27 09:58:06 | announced | |
2 | 2024-10-22 06:15:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proximity labeling, mitochondria,synaptic plasticity |
Contact List
Julian Langer |
contact affiliation | Max Planck Institute of Brain Research |
contact email | julian.langer@brain.mpg.de |
lab head | |
Julian Langer |
contact affiliation | MPIs for Biophysics and Brain Research |
contact email | julian.langer@biophys.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047226
- Label: PRIDE project
- Name: VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity