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PXD047226-1

PXD047226 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity
DescriptionSynapses are pivotal sites of plasticity and memory formation. Consequently, synapses are energy consumption hotspots susceptible to dysfunction when their energy supplies are perturbed. Mitochondria are stabilized near synapses via the cytoskeleton and provide the local energy required for synaptic plasticity. However, the mechanisms that tether and stabilize mitochondria to support synaptic plasticity are unknown. We identified proteins exclusively tethering mitochondria to actin near postsynaptic spines. We find that VAP, the vesicle-associated membrane protein-associated protein implicated in amyotrophic lateral sclerosis, stabilizes mitochondria via actin near the spines. To test if the VAP-dependent stable mitochondrial compartments can locally support synaptic plasticity, we used two-photon glutamate uncaging for spine plasticity induction and investigated the induced and adjacent uninduced spines. We find VAP functions as a spatial stabilizer of mitochondrial compartments for up to ~60 min and as a spatial ruler determining the ~30 m dendritic segment supported during synaptic plasticity.
HostingRepositoryPRIDE
AnnounceDate2023-11-27
AnnouncementXMLSubmission_2023-11-27_09:58:06.442.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulian Langer
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive; maXis; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-24 09:26:08ID requested
12023-11-27 09:58:06announced
22024-10-22 06:15:59announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proximity labeling, mitochondria,synaptic plasticity
Contact List
Julian Langer
contact affiliationMax Planck Institute of Brain Research
contact emailjulian.langer@brain.mpg.de
lab head
Julian Langer
contact affiliationMPIs for Biophysics and Brain Research
contact emailjulian.langer@biophys.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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