PXD047054 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics analysis of humulene-producing yeast integrated with a low temeprature-inducible GAL circuit for pathway induction |
Description | We analysed the proteome of two humulene-producing yeast strains under normal temperature (30 °C) cultivation condition and low temperature (25 °C) cultivation condition. In these two humulene-producing yeast strains, HUM401RU (control strain) and CI-HUM401RU (low-temperature-inducible strain), the mevalonate pathway genes and the humulene synthase gene are controlled by different galactose-inducible (GAL) promoters. For CI-HUM401RU, a low-temperature-inducible GAL circuit has been introduced to enable induction of pathway genes at 25 °C. For the control strain, HUM401RU, a tetracycline-inducible promoter is used to control GAL80, so that the pathway genes can be auto-induced after glucose depleted during the cultivation. We in total submitted 12 samples which can be devided into four groups: (1) HUM401RU, 25 °C, 24h; (2) CI-HUM401RU, 30 °C, 24h; (3) CI-HUM401RU, 25 °C, 24 h; (4) CI-HUM401RU, 25 °C, 48 h. Each group has three bilogical replites. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:54:22.320.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zeyu Lu |
SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-18 04:09:01 | ID requested | |
1 | 2024-08-10 12:17:43 | announced | |
⏵ 2 | 2024-10-22 06:54:27 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1093/nar/gkae460; |
Lu Z, Shen Q, Bandari NC, Evans S, McDonnell L, Liu L, Jin W, Luna-Flores CH, Collier T, Talbo G, McCubbin T, Esquirol L, Myers C, Trau M, Dumsday G, Speight R, Howard CB, Vickers CE, Peng B, LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae. Nucleic Acids Res, 52(12):7367-7383(2024) [pubmed] |
Keyword List
submitter keyword: humulene production, low-temperature cultivation,Saccharomyces cerevisiae, low-temperature-inducible GAL circuit |
Contact List
Zeyu Lu |
contact affiliation | The University of Queensland |
contact email | zeyu.lu@uq.net.au |
lab head | |
Zeyu Lu |
contact affiliation | The University of Queensland; Queensland University of Technology |
contact email | zeyu.lu@uq.net.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047054
- Label: PRIDE project
- Name: Proteomics analysis of humulene-producing yeast integrated with a low temeprature-inducible GAL circuit for pathway induction