PXD047054-1

PXD047054 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of humulene-producing yeast integrated with a low temeprature-inducible GAL circuit for pathway induction
Description	We analysed the proteome of two humulene-producing yeast strains under normal temperature (30 °C) cultivation condition and low temperature (25 °C) cultivation condition. In these two humulene-producing yeast strains, HUM401RU (control strain) and CI-HUM401RU (low-temperature-inducible strain), the mevalonate pathway genes and the humulene synthase gene are controlled by different galactose-inducible (GAL) promoters. For CI-HUM401RU, a low-temperature-inducible GAL circuit has been introduced to enable induction of pathway genes at 25 °C. For the control strain, HUM401RU, a tetracycline-inducible promoter is used to control GAL80, so that the pathway genes can be auto-induced after glucose depleted during the cultivation. We in total submitted 12 samples which can be devided into four groups: (1) HUM401RU, 25 °C, 24h; (2) CI-HUM401RU, 30 °C, 24h; (3) CI-HUM401RU, 25 °C, 24 h; (4) CI-HUM401RU, 25 °C, 48 h. Each group has three bilogical replites.
HostingRepository	PRIDE
AnnounceDate	2024-08-10
AnnouncementXML	Submission_2024-08-10_12:17:42.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zeyu Lu
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-18 04:09:01	ID requested
⏵ 1	2024-08-10 12:17:43	announced
2	2024-10-22 06:54:27	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkae460;

Lu Z, Shen Q, Bandari NC, Evans S, McDonnell L, Liu L, Jin W, Luna-Flores CH, Collier T, Talbo G, McCubbin T, Esquirol L, Myers C, Trau M, Dumsday G, Speight R, Howard CB, Vickers CE, Peng B, LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae. Nucleic Acids Res, 52(12):7367-7383(2024) [pubmed]

submitter keyword: humulene production, low-temperature cultivation,Saccharomyces cerevisiae, low-temperature-inducible GAL circuit

Zeyu Lu
contact affiliation	The University of Queensland
contact email	zeyu.lu@uq.net.au
lab head
Zeyu Lu
contact affiliation	The University of Queensland; Queensland University of Technology
contact email	zeyu.lu@uq.net.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047054
     1. Label: PRIDE project
     2. Name: Proteomics analysis of humulene-producing yeast integrated with a low temeprature-inducible GAL circuit for pathway induction