<<< Full experiment listing

PXD047054-1

PXD047054 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis of humulene-producing yeast integrated with a low temeprature-inducible GAL circuit for pathway induction
DescriptionWe analysed the proteome of two humulene-producing yeast strains under normal temperature (30 °C) cultivation condition and low temperature (25 °C) cultivation condition. In these two humulene-producing yeast strains, HUM401RU (control strain) and CI-HUM401RU (low-temperature-inducible strain), the mevalonate pathway genes and the humulene synthase gene are controlled by different galactose-inducible (GAL) promoters. For CI-HUM401RU, a low-temperature-inducible GAL circuit has been introduced to enable induction of pathway genes at 25 °C. For the control strain, HUM401RU, a tetracycline-inducible promoter is used to control GAL80, so that the pathway genes can be auto-induced after glucose depleted during the cultivation. We in total submitted 12 samples which can be devided into four groups: (1) HUM401RU, 25 °C, 24h; (2) CI-HUM401RU, 30 °C, 24h; (3) CI-HUM401RU, 25 °C, 24 h; (4) CI-HUM401RU, 25 °C, 48 h. Each group has three bilogical replites.
HostingRepositoryPRIDE
AnnounceDate2024-08-10
AnnouncementXMLSubmission_2024-08-10_12:17:42.435.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZeyu Lu
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-18 04:09:01ID requested
12024-08-10 12:17:43announced
22024-10-22 06:54:27announced2024-10-22: Updated project metadata.
Publication List
10.1093/nar/gkae460;
Lu Z, Shen Q, Bandari NC, Evans S, McDonnell L, Liu L, Jin W, Luna-Flores CH, Collier T, Talbo G, McCubbin T, Esquirol L, Myers C, Trau M, Dumsday G, Speight R, Howard CB, Vickers CE, Peng B, LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae. Nucleic Acids Res, 52(12):7367-7383(2024) [pubmed]
Keyword List
submitter keyword: humulene production, low-temperature cultivation,Saccharomyces cerevisiae, low-temperature-inducible GAL circuit
Contact List
Zeyu Lu
contact affiliationThe University of Queensland
contact emailzeyu.lu@uq.net.au
lab head
Zeyu Lu
contact affiliationThe University of Queensland; Queensland University of Technology
contact emailzeyu.lu@uq.net.au
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD047054
PRIDE project URI
Repository Record List
[ + ]