PXD046601-2

PXD046601 is an original dataset announced via ProteomeXchange.

Title	20210901_WXY_TMT_2plex_3Rep_Huvec_proteins
Description	Cells were harvested with intra-cellular proteins extracted and digested with trypsin. Peptides were then labeled with TMT using a TMT10plex mass tag labeling kit (Thermo Fisher Scientific) per the manufacturer’s instructions. TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was subsequently performed. All fragments were sequentially dissolved in aqueous solution (0.1% formic acid and 2% acetonitrile), loaded onto a home-made reverse-phase analytical column, and subjected to an EASY-nLC™ 1000 ultraperformance liquid chromatography (UPLC) system (Thermo Fisher Scientific) at a constant flow rate of 400 nL/minutes. For MS settings, the applied electrospray voltage was 2.0 kV and the m/z range was 350 to 1800 for the complete scan. Peptide fragments were quantified with Parallel Reaction Monitoring (PRM). Proteins were identified by Swissprot database and quantified using Proteome Discoverer 2.0.
HostingRepository	iProX
AnnounceDate	2023-11-01
AnnouncementXML	Submission_2024-03-06_00:16:17.962.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; ACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-01 20:32:08	ID requested
1	2024-01-28 22:50:35	announced
⏵ 2	2024-03-06 00:16:19	announced	2024-03-06: Update information.

Chen X, Wang Y, Wang JN, Zhang YC, Zhang YR, Sun RX, Qin B, Dai YX, Zhu HJ, Zhao JX, Zhang WW, Ji JD, Yuan ST, Shen QD, Liu QH, Lactylation-driven FTO targets CDK2 to aggravate microvascular anomalies in diabetic retinopathy. EMBO Mol Med, 16(2):294-318(2024) [pubmed]

submitter keyword: Proteomic analyses, HUVECs, FTO

Xue Chen
contact affiliation	Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University
contact email	drcx1990@vip.163.com
lab head
Ying Wang
contact affiliation	Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University
contact email	wangying60131@163.com
dataset submitter

iProX dataset URI