PXD046601-1
PXD046601 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | 20210901_WXY_TMT_2plex_3Rep_Huvec_proteins |
Description | Cells were harvested with intra-cellular proteins extracted and digested with trypsin. Peptides were then labeled with TMT using a TMT10plex mass tag labeling kit (Thermo Fisher Scientific) per the manufacturer’s instructions. TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was subsequently performed. All fragments were sequentially dissolved in aqueous solution (0.1% formic acid and 2% acetonitrile), loaded onto a home-made reverse-phase analytical column, and subjected to an EASY-nLC™ 1000 ultraperformance liquid chromatography (UPLC) system (Thermo Fisher Scientific) at a constant flow rate of 400 nL/minutes. For MS settings, the applied electrospray voltage was 2.0 kV and the m/z range was 350 to 1800 for the complete scan. Peptide fragments were quantified with Parallel Reaction Monitoring (PRM). Proteins were identified by Swissprot database and quantified using Proteome Discoverer 2.0. |
HostingRepository | iProX |
AnnounceDate | 2023-11-01 |
AnnouncementXML | Submission_2024-01-28_22:50:35.230.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ying Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; ACQUITY UPLC |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-11-01 20:32:08 | ID requested | |
⏵ 1 | 2024-01-28 22:50:35 | announced | |
2 | 2024-03-06 00:16:19 | announced | 2024-03-06: Update information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Proteomic analyses, HUVECs, FTO |
Contact List
Xue Chen | |
---|---|
contact affiliation | Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University |
contact email | drcx1990@vip.163.com |
lab head | |
Ying Wang | |
contact affiliation | Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University |
contact email | wangying60131@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |