PXD046601-1
PXD046601 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | 20210901_WXY_TMT_2plex_3Rep_Huvec_proteins |
| Description | Cells were harvested with intra-cellular proteins extracted and digested with trypsin. Peptides were then labeled with TMT using a TMT10plex mass tag labeling kit (Thermo Fisher Scientific) per the manufacturer’s instructions. TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was subsequently performed. All fragments were sequentially dissolved in aqueous solution (0.1% formic acid and 2% acetonitrile), loaded onto a home-made reverse-phase analytical column, and subjected to an EASY-nLC™ 1000 ultraperformance liquid chromatography (UPLC) system (Thermo Fisher Scientific) at a constant flow rate of 400 nL/minutes. For MS settings, the applied electrospray voltage was 2.0 kV and the m/z range was 350 to 1800 for the complete scan. Peptide fragments were quantified with Parallel Reaction Monitoring (PRM). Proteins were identified by Swissprot database and quantified using Proteome Discoverer 2.0. |
| HostingRepository | iProX |
| AnnounceDate | 2023-11-01 |
| AnnouncementXML | Submission_2024-01-28_22:50:35.230.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ying Wang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF; ACQUITY UPLC |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-01 20:32:08 | ID requested | |
| ⏵ 1 | 2024-01-28 22:50:35 | announced | |
| 2 | 2024-03-06 00:16:19 | announced | 2024-03-06: Update information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Proteomic analyses, HUVECs, FTO |
Contact List
| Xue Chen | |
|---|---|
| contact affiliation | Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University |
| contact email | drcx1990@vip.163.com |
| lab head | |
| Ying Wang | |
| contact affiliation | Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University |
| contact email | wangying60131@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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