PXD046345-1

PXD046345 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Formyl-methionine as an early elongational ribosome destabilization signal
Description	Despite recent findings of cytosolic fMet-protein translation in eukaryotes, the underlying mechanism that limits cytosolic fMet-protein translation remains unknown. Here, we found that fMet-nascent peptide signals premature ribosome dissociation in S. cerevisiae, that we named N-terminal fMet-initiated ribosome quality control (fMet-RQC). pBpa-dependent photo-crosslinking of stalled fMet-nascent peptide co-purified with Nip1 and Arf1/2, suggesting their functional importance in fMet-RQC pathway. Defective fMet-RQC leads to co-translational fMet-protein aggregation hindering cellular stress adaptiveness. We also found that dynamics of fMet-RQC is critical in regulating cytosolic fMet-protein level during extreme environmental stresses, such as prolonged cold stress, for cellular survival.
HostingRepository	jPOST
AnnounceDate	2024-10-24
AnnouncementXML	Submission_2024-10-23_08:00:06.986.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046345
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ChangSeok Lee
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; N-formyl-L-proline; L-methionine sulfoxide
Instrument	Q Exactive Plus; Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 23:40:43	ID requested
⏵ 1	2024-10-23 08:00:07	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: N-terminal formylation, ribosome quality control, translation regulation, stress adaptation

submitter keyword: N-terminal formylation, ribosome quality control, translation regulation, stress adaptation

Contact List

Cheol-Sang Hwang
lab head
ChangSeok Lee
contact affiliation	POSTECH
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002352/