PXD045881-3

PXD045881 is an original dataset announced via ProteomeXchange.

Title	Alterations in the extracellular proteome of human pathogen Pseudomonas aeruginosa in response to natural and modified N-acyl-L-homoserine lactones
Description	The common opportunistic human pathogen Pseudomonas aeruginosa employs quorum sensing QS to regulate a large set of genes involved in virulence and host-pathogen interactions. The Las circuit positioned on the top of the QS hierarchy in P. aeruginosa, makes use of N-acyl-L-homoserine lactones (AHL) as signal molecules, like N-3-oxo-dodecanoyl-L-homoserine lactone (3O-C12-HSL). Here, a quantitative proteomic approach was used to study the effect of natural 3O-C12-HSL and four AHL analogues on the expression and excretion of QS-regulated extracellular proteins. Treatment with AHL compounds resulted in significant difference in appearance of the 3O-C12-HSL-responsive reference proteins related to QS communication and virulence, i.e., a distinct activity as QS modulators. In summary, 3O-C12-HSL has a profound effect on extracellular proteome involved in the pathogenicity of P. aeruginosa, and the four AHL analogues have achieved a distinct inhibitory effect on the extracellular proteome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:07.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Vikström
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-03 10:43:38	ID requested
1	2023-10-06 07:22:17	announced
2	2023-11-14 08:10:37	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:05:08	announced	2024-10-22: Updated project metadata.

10.3389/FMOLB.2023.1264773;

submitter keyword: Pseudomons aeruginosa

extracellular proteome

lactone

Elena Vikström
contact affiliation	Linkoping University
contact email	elena.vikstrom@liu.se
lab head
Elena Vikström
contact affiliation	Linköping University
contact email	elena.vikstrom@liu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045881

PRIDE project URI

• PRIDE
  1. PXD045881
     1. Label: PRIDE project
     2. Name: Alterations in the extracellular proteome of human pathogen Pseudomonas aeruginosa in response to natural and modified N-acyl-L-homoserine lactones