PXD045881-2

PXD045881 is an original dataset announced via ProteomeXchange.

Title	Alterations in the extracellular proteome of human pathogen Pseudomonas aeruginosa in response to natural and modified N-acyl-L-homoserine lactones
Description	The common opportunistic human pathogen Pseudomonas aeruginosa employs quorum sensing QS to regulate a large set of genes involved in virulence and host-pathogen interactions. The Las circuit positioned on the top of the QS hierarchy in P. aeruginosa, makes use of N-acyl-L-homoserine lactones (AHL) as signal molecules, like N-3-oxo-dodecanoyl-L-homoserine lactone (3O-C12-HSL). Here, a quantitative proteomic approach was used to study the effect of natural 3O-C12-HSL and four AHL analogues on the expression and excretion of QS-regulated extracellular proteins. Treatment with AHL compounds resulted in significant difference in appearance of the 3O-C12-HSL-responsive reference proteins related to QS communication and virulence, i.e., a distinct activity as QS modulators. In summary, 3O-C12-HSL has a profound effect on extracellular proteome involved in the pathogenicity of P. aeruginosa, and the four AHL analogues have achieved a distinct inhibitory effect on the extracellular proteome.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:10:37.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Vikström
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-03 10:43:38	ID requested
1	2023-10-06 07:22:17	announced
⏵ 2	2023-11-14 08:10:37	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:05:08	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Pseudomons aeruginosa

extracellular proteome

lactone

Elena Vikström
contact affiliation	Linkoping University
contact email	elena.vikstrom@liu.se
lab head
Elena Vikström
contact affiliation	Linköping University
contact email	elena.vikstrom@liu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045881
     1. Label: PRIDE project
     2. Name: Alterations in the extracellular proteome of human pathogen Pseudomonas aeruginosa in response to natural and modified N-acyl-L-homoserine lactones